Aquilegia Physical Map
High information content fingerprinting (HICF) technique, based on capillary electrophoresis and the SNaPshot labeling kit (Luo et al., 2003. Genomics, 82(3): 378-389), is used to construct a physical map for A. formosa. Both BAC libraries of A. formosa, one (AF__Bb) consructed using HindIII enzyme and the other (AF__Bc) using MboI enzyme, will be combined into a single physical map. Click the tab above to view the current releases of the A. formosa physical map using WebFPC.
Total Clones: 52,554, Contigs: 3,972, Markers: 1, Singletons: 5,939
Physical Map Release Date: Jan 25, 2006 (third release)
The evolution of life on earth has been punctuated by numerous examples of adaptive radiation. These dramatic events quickly create a large amount of biodiversity and are evidenced by rapid speciation along with morphological and physiological adaptations to numerous ecological niches. Species in the flowering plant genus Aquilegia have undergone a very recent adaptive radiation and present a unique opportunity to investigate the molecular genetic changes underlying adaptations. Species in this genus have spectacularly different floral morphologies with specializations to different pollinators. In addition, species differ radically in their habitats ranging from coastal forests to desert springs to the high alpine. Because any two species in the genus can be successfully crossed it is possible to dissect the genetic basis for essentially any trait in any species. By developing an array of molecular genetic resources for this genus, this project will provide the infrastructure for a host of studies by a broad community of scientists. All of these studies will be facilitated by the fact that the genome of Aquilegia is among the smallest for a flowering plant at about 350 Mbp. The specific goals of this project are to evaluate the genetic basis of three important traits, morphological adaptation to a specific pollinator, physiological adaptation for flowering time and adaptation to different soil/habitats.
